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more generic term "GOI" representing inserted "gene of interest").
(5′-NTR) retained the viral ribosomal entry site to initiate translation of the Npro gene and GOI; insertion of the GOI was provided
IRES) was engineered downstream of the GOI insertion site for
Npro gene fused in frame to the GOI terminated with a stop codon,
for inserting as GOI that encoding influenza virus hemagglutinin
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